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Identification of Plum pox virus Determinants Implicated in Specific Interactions with Different Prunus spp.

February 2001 , Volume 91 , Number  2
Pages  159 - 164

Sylvie Dallot , Laurence Quiot-Douine , Pilar Sáenz , Maria Teresa Cervera , Juan-Antonio García , and Jean-Bernard Quiot

First, second, and sixth authors: Equipe virus-vecteur, ENSA-INRA, 2 place Viala, 34060 Montpellier cedex 1, France; and third, fourth, and fifth authors: Centro Nacional de Biotecnología, Campus de la Universidad Autónoma de Madrid, 28049 Spain

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Accepted for publication 17 October 2000.

The characterization of pathogenic properties of two infectious clones of Plum pox virus (PPV) isolates, pGPPV (D group) and pGPPVPS (M group), was investigated in their woody hosts (seedlings of Prunus spp.). The two clones differed in their ability to infect plum and peach cultivars, from no infection to local and systemic infection. The phenotype determinants were located with a set of chimeric viruses from the two clones. In plum, determinants of systemic infection were located in a genomic fragment encoding the P3 and 6K1 proteins, which might influence genome amplification or virus movement. The capacity of pGPPVPS to induce stable local and systemic infections in peach was not located accurately and might be influenced by multiple determinants carried by different regions of the genome, excluding those encoding the protein 1, the majority of helper component, nuclear inclusions a and b, and coat protein. We conclude that PPV infections of plum and peach are governed by different determinants.

The American Phytopathological Society, 2001