Transcriptomic, proteomic, and nutritional analyses of potato tissues infected with ‘Candidatus Liberibacter solanacearum’
C. NWUGO (1), V. Venkatesan (2), J. Munyaneza (2), H. Lin (1). (1) USDA-ARS, Parlier, CA, U.S.A.; (2) USDA-ARS, Austin, TX, U.S.A.
Zebra chip (ZC) is an emerging destructive disease of potato and is associated with the phloem-limited α-proteobacterium, ‘<i>Candidatus</i> Liberibacter solanacearum’ (Lso). In this study, RNA-Seq, 2-DE, mass spectrometry and qRT-PCR analyses identified over 100 differentially produced gene transcripts and proteins in above-ground (AG) and below-ground (BG) potato tissues upon Lso infection. Interestingly, in spite of an Lso-mediated down-regulation of photosynthesis-related genes/proteins, over 80% of the differentially produced gene transcripts/proteins were up-regulated in AG tissues. This was accompanied by an increase in nutrient concentrations of K, Mn, Fe and Cu in both AG and BG tissues in response to Lso infection. Furthermore, there was a strong induction of proteinase inhibitors in AG tissues upon Lso infection. In contrast, the expression of proteinase inhibitors was markedly suppressed in Lso-infected BG tissues. In general, results suggest that ZC disease development involves an Lso-mediated down-regulation of photosynthesis accompanied by an ineffective and potentially inefficient up-regulation of stress response-, metabolism-, and housekeeping-related gene transcripts/proteins with concomitant increases in nutrient accumulation. This study presents a first approach of a holistic investigation of the global transcriptomic, proteomic and nutritional response of potato plants to Lso infection.
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