Mating of Aspergillus flavus x Aspergillus minisclerotigenes hybrids: Are they functionally mules?
K. DAMANN (1), C. DeRobertis (1). (1) Louisiana State University Agricultural Center, Baton Rouge, LA, U.S.A.
We previously demonstrated sexual reproductive ability between isolates of <i>A. flavus</i> (horses 5, 26) and <i>A. minisclerotigenes</i> (donkeys 13, 28). Ten sexual hybrid progeny (mules) from each of two crosses (5x28, 26x13) were used in this study. The 10 F1’s of 5x28 were crossed with their opposite mating type parent. Two of 10 produced viable ascospore progeny (mules x horses). The 10 F1’s of 26x13 were crossed with their opposite mating type parent. Four of 10 produced viable ascospore progeny (mules x horses). The 10 F1’s of 5x28 were distributed 7 <i>Mat1-1 to 3 Mat1-2</i> allowing 21 within group crosses. Four of 21 produced viable ascospore progeny (mules x mules). The 10 F1’s of 26x13 were distributed 8 <i>Mat1-1</i> to 2 <i>Mat1-2</i> allowing 16 within group crosses. Four of the 16 produced viable ascospore progeny (mules x mules). The frequency of successful matings with hybrids as parents approximates those within and between <i>A. flavus</i> and <i>A. minisclerotigenes</i>, however no successful matings occurred between the hybrid mules and <i>A. minisclerotigenes</i> donkeys.
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