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Nutrient Broth Yeast extract Medium (NBY) (12)

Per liter add:

 

   nutrient broth

8 g

   yeast extract

2 g

   K2HPO4 (anhydrous)

2 g

   KH2PO4

0.5 g

   Distilled H2O

974 ml

For NBY agar add:

 

   Agar

15 g

Add after autoclaving

 

    20% glucose

25 ml

    1 M MgSO4

1 ml

SOC Medium (10)

Per liter add:

 

   Bacto-tryptone

20 g

   Bacto-yeast extract

5 g

   NaCl

0.5 g

   250 mM KCl

10 ml

   Distilled H2O

970 ml

Adjust the pH to 7.0 with 5 N NaOH

~0.2 ml

Add after autoclaving

 

   1 M MgCl2

5 ml

   1 M glucose

25 ml