Nutrient Broth Yeast extract Medium (NBY) (12)
|
Per liter add: |
|
|
nutrient broth |
8 g |
|
yeast extract |
2 g |
|
K2HPO4 (anhydrous) |
2 g |
|
KH2PO4 |
0.5 g |
|
Distilled H2O |
974 ml |
|
For NBY agar add: |
|
|
Agar |
15 g |
|
Add after autoclaving |
|
|
20% glucose |
25 ml |
|
1 M MgSO4 |
1 ml |
SOC Medium (10)
|
Per liter add: |
|
|
Bacto-tryptone |
20 g |
|
Bacto-yeast extract |
5 g |
|
NaCl |
0.5 g |
|
250 mM KCl |
10 ml |
|
Distilled H2O |
970 ml |
|
Adjust the pH to 7.0 with 5 N NaOH |
~0.2 ml |
|
Add after autoclaving |
|
|
1 M MgCl2 |
5 ml |
|
1 M glucose |
25 ml |
