Craig G. Webster, United States Department of Agriculture-Agricultural Research Service (USDA-ARS), U.S. Horticultural Research Laboratory, Fort Pierce, FL 34945 USA;
Chandrasekar S. Kousik, USDA-ARS, U.S. Vegetable Laboratory, Charleston, SC 29414 USA;
William W. Turechek, USDA-ARS, U.S. Horticultural Research Laboratory, Fort Pierce, FL 34945 USA;
Susan E. Webb, University of Florida, Department of Entomology and Nematology, Gainesville, FL 32611 USA;
Pamela D. Roberts, Department of Plant Pathology, Southwest Florida Research and Education Center, University of Florida, Immokalee, FL 34142 USA; and
Scott Adkins, USDA-ARS, U.S. Horticultural Research Laboratory, Fort Pierce, FL 34945 USA
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Accepted for publication 13 March 2013.
The responses of a diverse group of vining cucurbits to inoculation with Squash vein yellowing virus (SqVYV) were determined. For the first time, Cucurbita maxima, Cucumis dipsaceus, and Cucumis metuliferus were observed to develop necrosis and plant death similar to the SqVYV-induced vine decline in watermelon (Citrullus lanatus var. lanatus). The majority of cucurbits inoculated, however, either exhibited no symptoms of infection, or developed relatively mild symptoms such as vein yellowing of upper, noninoculated leaves. All inoculated plants were sectioned and tested for the presence of SqVYV. The virus was widely distributed in mature, fruit-bearing cucurbits with over 72% of plant sections testing positive for SqVYV by tissue-blot and/or reverse transcription-polymerase chain reaction. Plants of several cucurbits, including a wild citron (Citrullus lanatus var. citroides), were symptomless and had a decreased frequency of virus infection of vine segments compared to susceptible vining cucurbits, indicating a higher level of resistance. However, no significant relationship between the frequency of infection or virus distribution within plants and the symptom response was observed. These results demonstrate that a diverse group of cucurbits may decline when infected with SqVYV, and suggest that widespread distribution of virus within the plant is not the sole cause of decline.
This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 2013.