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Monitoring Current-Season Spread of Potato virus Y in Potato Fields Using ELISA and Real-Time RT-PCR

May 2013 , Volume 97 , Number  5
Pages  641 - 644

Manphool S. Fageria and Mathuresh Singh, Agricultural Certification Services, Fredericton, NB, Canada E3B 8B7; Upeksha Nanayakkara, Yvan Pelletier and Xianzhou Nie, Potato Research Centre, Agriculture and Agri-Food Canada, Fredericton, NB, Canada E3B 4Z7; and David Wattie, New Brunswick Department of Agriculture, Aquaculture and Fisheries, Wicklow, NB, Canada E7L 3S4

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Accepted for publication 4 December 2012.

The current-season spread of Potato virus Y (PVY) was investigated in New Brunswick, Canada, in 11 potato fields planted with six different cultivars in 2009 and 2010. In all, 100 plants selected from each field were monitored for current-season PVY infections using enzyme-linked immunosorbent assay (ELISA) and real-time reverse-transcription polymerase chain reaction (RT-PCR) assay. Average PVY incidence in fields increased from 0.6% in 2009 and 2% in 2010 in the leaves to 20.3% in 2009 and 21.9% in 2010 in the tubers at the time of harvest. In individual fields, PVY incidence in tubers reached as high as 37% in 2009 and 39% in 2010 at the time of harvest. Real-time RT-PCR assay detected more samples with PVY from leaves than did ELISA. A higher number of positive samples was also detected with real-time RT-PCR from growing tubers compared with the leaves collected from the same plant at the same sampling time. PVY incidence determined from the growing tubers showed a significant positive correlation with the PVY incidence of tubers after harvest. Preharvest testing provides another option to growers to either top-kill the crop immediately to secure the seed market when the PVY incidence is low or leave the tubers to develop further for table or processing purposes when incidence of PVY is high.

© 2013 The American Phytopathological Society