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Molecular Variation of Sporisorium scitamineum in Mainland China Revealed by RAPD and SRAP Markers

October 2012 , Volume 96 , Number  10
Pages  1,519 - 1,525

Y. Que, L. Xu, J. Lin, and R. Chen, Key Lab of Sugarcane Genetic Improvement, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, China; and M. P. Grisham, United States Department of Agriculture–Agricultural Research Service, Sugarcane Research Unit, Houma, LA 70360

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Accepted for publication 3 May 2012.

Sugarcane smut caused by Sporisorium scitamineum occurs worldwide, causing serious losses in sugar yield and quality. To study the molecular variation of S. scitamineum, 23 S. scitamineum isolates collected from the six primary sugarcane production areas in mainland China (Guangxi, Yunnan, Guangdong, Hainan, Fujian, and Jiangxi provinces) were assessed by random amplified polymorphic DNA (RAPD) and sequence-related amplified polymorphism (SRAP) markers. The results of RAPD, SRAP, and RAPD-SRAP combined analysis showed that, whereas the molecular variation of S. scitamineum was associated with geographic origin, there was no evidence of co-evolution between sugarcane and the pathogen. The results of RAPD, SRAP, or RAPD-SRAP combined analysis also did not provide any information about race differentiation of S. scitamineum. This suggests that the mixture of spores from sori collected from different areas should be used in artificial inoculations for resistance breeding and selection.

© 2012 The American Phytopathological Society