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Genetic Transformation of Passionflower and Evaluation of R1 and R2 Generations for Resistance to Cowpea aphid borne mosaic virus

August 2011 , Volume 95 , Number  8
Pages  1,021 - 1,025

Alessandra C. B. A. Monteiro-Hara, Laboratório de Biotecnologia Vegetal, Centro de Energia Nuclear na Agricultura, Universidade de São Paulo (CENA/USP), 13400-970 Piracicaba, SP, Brazil; Adriana S. Jadão, Departamento de Fitopatologia e Nematologia, Escola Superior de Agricultura ‘Luiz de Queiroz’, Universidade de São Paulo (ESALQ/USP), 13418-900 Piracicaba, SP, Brazil; Beatriz M. J. Mendes, Laboratório de Biotecnologia Vegetal, CENA/USP; Jorge A. M. Rezende, Departamento de Fitopatologia e Nematologia, ESALQ/USP; Flavio Trevisan, Laboratório de Biotecnologia Vegetal, CENA/USP; Ana Paula O. A. Mello, Departamento de Fitopatologia e Nematologia, ESALQ/USP; Maria Lucia C. Vieira, Departamento de Genética, ESALQ/USP, 13418-900 Piracicaba, SP, Brazil; L. M. M. Meletti, Centro de Fruticultura, Instituto Agronômico, 13020-902 Campinas, SP, Brazil; and Sônia M. De S. Piedade, Departamento de Ciências Exatas, ESALQ/USP

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Accepted for publication 6 April 2011.

We report on the production and evaluation of passionflower transgenic lines for resistance to Cowpea aphid borne mosaic virus (CABMV). Genetic transformation was done using Agrobacterium tumefaciens and transgene integration was confirmed by Southern blot analyses, resulting in nine transgenic lines for ‘IAC 275’ and three for ‘IAC 277’. Transgenic lines were clonally propagated and evaluated for resistance to CABMV. After the third inoculation, under higher inoculum pressure, only propagated plants of the transgenic line T16 remained asymptomatic, indicating a high resistance to infection with CABMV. This transgenic line was self-pollinated and the R1 generation was evaluated together with the R1 generation of another resistant transgenic line (T2) identified previously. Plants were inoculated with CABMV by means of viruliferous Myzus nicotianae. All 524 T2R1 plants became infected, whereas 13 of 279 T16R1 remained asymptomatic after four successive inoculations. A T16R2 generation was obtained and plants were inoculated with CABMV mechanically or by aphids. After successive inoculations, 118 of 258 plants were symptomless, suggesting that the resistance to CABMV was maintained in the plant genome as the homozygous condition was achieved. Five selected resistant T16R2 plants which contained the capsid protein gene are being crossed for further analyses.

© 2011 The American Phytopathological Society