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Phytophthora taxon niederhauserii, a New Root and Crown Rot Pathogen of Banksia spp. in Italy

November 2009 , Volume 93 , Number  11
Pages  1,216.3 - 1,217

S. O. Cacciola and S. Scibetta, Dipartimento di Chimica Biologica, Chimica Medica e Biologia Molecolare, University of Catania, 95125 Catania, Italy; P. Martini, Istituto Regionale per la Floricoltura, 18038 Sanremo, Italy; C. Rizza, Dipartimento di Scienze Entomologiche, Fitopatologiche, Microbiologiche Agrarie e Zootecniche, University of Palermo, 90128 Palermo; and A. Pane, Dipartimento di Scienze e Tecnologie Fitosanitarie, University of Catania, 95123 Catania, Italy

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Accepted for publication 5 August 2009.

In the last 10 years, various species of Banksia (family Proteaceae) endemic to Australia have been introduced into Italy where cultivation as flower plants is expanding. In the spring of 2003, a decline associated with root and basal stem rot of 2- to 3-year-old plants of Banksia speciosa R. Br., B. baxteri R. Br., and B. prionotes Lindl. grown in the ground was observed in a commercial nursery in Liguria (northern Italy). Aboveground symptoms included leaf chlorosis and wilt. Plants collapsed within 1 to 2 weeks after the appearance of leaf symptoms. A Phytophthora species was consistently isolated from roots and basal stem on BNPRAH selective medium (3). On V8 juice agar (V8A), axenic cultures obtained by single hyphal transfers formed stellate to radiate colonies with aerial mycelium; on potato dextrose agar (PDA). the colonies showed stoloniform mycelium. Minimum and maximum growth temperatures on PDA and V8A were between 5 and 10°C and 38 and 40°C, respectively, with the optimum at 30°C on PDA (mean radial growth rate of 10 isolates ranged between 9.3 and 10.2 mm per day) and 25 to 30°C on V8A (14 mm per day). In saline solution and soil extract, all isolates produced catenulate hyphal swellings and ellipsoid, nonpapillate, persistent sporangia. Sporangia in saline solution varied from 47 to 70 × 30 to 44 μm (mean l/b ratio of 1.5). All isolates were A1 mating type and produced oogonia with amphyginous antheridia when paired with A2 mating type of P. drechsleri Tucker on V8A plus β-sytosterol (3). The electrophoretic patterns of total mycelial proteins and two isozymes (esterase and malate dehydrogenase) (2) of all isolates from Banksia plants were identical, but distinct from the patterns of isolates of other Phytophthora species, including P. drechsleri, P. megasperma sensu stricto, and P. sojae. Internal transcribed spacer (ITS) regions of rDNA were amplified with primers ITS4/ITS6 and sequences of two isolates, IMI 393960 from B. speciosa and 466/03 from B. baxteri (GenBank Nos. FJ648808 and FJ648809), were 100% identical to sequences of isolates identified as Phytophthora taxon niederhauserii Z. G. Abad and J. A. Abad (GenBank Nos. AY550916, AM942765, and EU244850). Pathogenicity tests were performed on 1-year-old potted plants of B. speciosa with isolates IMI 393960 and 466/03. Twenty plants per each isolate were transplanted into 12-cm-diameter pots containing infested soil prepared by mixing steam-sterilized sandy loam soil with 1% of inoculum produced on autoclaved wheat kernels. Twenty control plants were grown in autoclaved soil mix. Plants were kept in the greenhouse with natural light at 25 ± 2°C and watered to field capacity weekly. All Banksia plants transplanted in infested soil showed symptoms of wilt, leaf chlorosis, and basal stem rot within 2 to 3 weeks. Noninoculated plants remained healthy. P. taxon niederhauserii was reisolated solely from inoculated plants. P. taxon niederhauserii has been reported recently from Banksia spp. in Australia (1), but to our knowledge this is the first report from Italy. P. taxon niederhauserii may represent a threat to the cultivation of many ornamentals including Cystus spp., English ivy, and laurel (4).

References: (1) T. I. Burgess et al. Plant Dis. 93:215, 2009. (2) S. O. Cacciola et al. EPPO Bull. 20:47, 1990. (3) D. C. Erwin and O. K. Ribeiro. Phytophthora Diseases Worldwide. The American Phytopathological Society, St. Paul, MN, 1996. (4) E. Moralejo et al. Plant Pathol, 58:100, 2009.

© 2009 The American Phytopathological Society