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Identification of Variants of the High Plains virus Infecting Wheat in Kansas

December 2009 , Volume 93 , Number  12
Pages  1,265 - 1,274

Dallas L. Seifers, Kansas State University, Agricultural Research Center--Hays, Hays, KS 67601-9228; T. J. Martin, Kansas State University, Agricultural Research Center--Hays, Hays, KS 67601-9228; Tom L. Harvey, Department of Entomology, Kansas State University, Manhattan, KS 66506; S. Haber, Cereal Research Centre, Agriculture & Agri-Food Canada, Winnipeg, MB, Canada; O. Krokhin, V. Spicer, S. Ying, and K. G. Standing, Department of Physics and Astronomy, University of Manitoba, Winnipeg, MB R3T2N2, Canada

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Accepted for publication 20 July 2009.

The properties of two virus isolates (U04-82 and U04-83) obtained from two wheat (Triticum aestivum) plants expressing mosaic symptoms were investigated using enzyme-linked immunosorbent assay (ELISA), sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), time-of-flight mass spectrometry (TOFMS), and infection of wheat with resistance to Wheat streak mosaic virus (WSMV). The coat protein mass was estimated by SDS-PAGE as approximately 32 kDa for U04-82 and 30 kDa for U04-83. The amino acid sequence of the coat protein of U04-82 was 99.6 and 85.5% identical to two isolates, ABC58222 and TX96, respectively, of High Plains virus (HPV) described from Texas. U04-82 was transmitted by wheat curl mites and caused significant yield reductions in wheat resistant to WSMV. U04-83 was actually two distinct virus isolates whose capsid protein amino acid sequences were only 57 and 50% similar to that of TX96. Antiserum prepared to a synthetic peptide from the sequence of the U04-83 isolate recognized the two U04-83 isolates, but not the U04-82 isolate.

© 2009 The American Phytopathological Society