Authors
S. L.
Gieck
,
Hermiston Agricultural Research and Extension Center (HAREC), Oregon State University, Hermiston 97838
;
H. R.
Pappu
,
Department of Plant Pathology, Washington State University, Pullman 99164
; and
P. B.
Hamm
and
N. L.
David
,
HAREC, Oregon State University, Hermiston, 97838
A general mosaic and yellowing of leaves of three cultivars of garlic (Allium sativum L., Late, Early, and Germinador) were observed in two seed-production fields in Morrow County, OR in June 2005. Approximately 50% of plants within the 50-ha fields were symptomatic. With recent findings of Onion yellow dwarf virus (OYDV), Leek yellow stripe virus (LYSV), and Garlic common latent virus (GCLV) in Washington (2), 45 composite samples of 10 leaves each from symptomatic (mosaic and yellowing) and nonsymptomatic plants were analyzed with a GCLV-specific antiserum (Agdia Inc., Elkhart, IN). All samples of ‘Germinador’ were infected regardless of symptoms, whereas 6.7% of all ‘Late’ and ‘Early’ samples were positive. GCLV infection was verified by reverse transcription (RT)-PCR using primers specific to the coat protein gene of GCLV followed by cloning and sequencing of the cloned amplicon. To determine the presence of a potyvirus, all composite samples were also tested with a general potyvirus antiserum (Agdia) and all samples from symptomatic plants were found to be positive. Representative positive samples from each cultivar were then tested by RT-PCR using degenerate, potyvirus group specific primers (3), and an amplicon of the expected size was obtained. To confirm which potyvirus was present, amplicons were cloned and sequenced, and sequence comparisons indicated that the representative samples were infected with OYDV. All symptomatic samples from the three cultivars were positive for OYDV when tested by RT-PCR using primers specific to its coat protein gene (1). Additionally, 53.3 and 6.7% of ‘Early’ and ‘Late’ samples, respectively, were also positive when tested with LYSV-specific primers (4). LYSV infection was further verified through cloning and sequencing of the cloned amplicon. Because this garlic is grown for seed, studies are being initiated to determine if current season spread occurs and yields are reduced. To our knowledge, this is the first report of OYDV, LYSV, and GCLV in garlic in Oregon.
References: (1) P. Lunello et al. J. Virol. Methods 118:15, 2004. (2) H. R. Pappu et al. Plant Dis. 89:205, 2005. (3) S. S. Pappu et al. J. Virol. Methods 41:9, 1993. (4) T. Tsuneyoshi et al. Phytopathology. 86:253, 1996.
