Department of Plant Pathology, The Pennsylvania State University, University Park 16802-4506
Bureau of Plant Industry, Pennsylvania Department of Agriculture, Harrisburg 17110-9408
Pythium isolates from commercial greenhouses in Pennsylvania were tested in vitro and in vivo for sensitivity to propamocarb. Isolates of Pythium aphanidermatum, P. irregulare, and P. ultimum consistently infected geranium seedlings that had been treated with propamocarb. P. ultimum and P. irregulare isolates exhibited dual fungicide resistance by overcoming propamocarb, mefenoxam, and a mixture of propamocarb and mefenoxam to infect seedlings. The sensitivity of isolates to propamocarb in vitro was not a good predictor of in vivo sensitivity. This is the first report of Pythium spp. resistance to propamocarb and dual resistance to propamocarb and mefenoxam.