Authors
F.
Fukumoto
,
National Agricultural Research Center, Department of Rice Research, Joetu, Inada, Niigata 943-0193 Japan
;
Y.
Masuda
,
Wakayama Research Center of Agriculture, Forestry and Fisheries, Agricultural Experiment Station, Takao, Kishigawa, Naga, Wakayama 640-0423 Japan
; and
K.
Hanada
,
National Agricultural Research Center for Kyushu Okinawa Region, Nishigoshi, Kikuchi, Kumamoto 861-1192 Japan
ABSTRACT
Necrotic diseases of the stems, petioles, and leaves of pea plants (Pisum sativumL.), leading to wilting and death, occur in the Wakayama and Mie Prefectures of Japan. Based on host range, symptomatology, electron microscopy, and serological relationships, Watermelon mosaic virus (WMV) and three Cucumber mosaic virus (CMV) isolates (PE2, PE3A, and PB1) were isolated from diseased plants in the Wakayama Prefecture. In the Mie Prefecture, CMV (PEAN) also was isolated from pea plants with similar symptoms. Single infection with CMV (PB1 or PEAN) caused stem necrosis and eventual death of pea plants. Similar symptoms developed after double infection with WMV and PE2 or PE3A, whereas single infection with PE2 and PE3A induced symptomless infection in pea plants. We concluded either CMV alone or synergistic effects of mixed infection with CMV and WMV induced pea plant stem necrosis.
