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RT-PCR Detection of Seedborne Cowpea aphid-borne mosaic virus in Peanut

November 2001 , Volume 85 , Number  11
Pages  1,181 - 1,182

A. G. Gillaspie , Jr. , USDA-ARS, Plant Genetic Resources Conservation Unit, Griffin, GA 30223 ; G. Pio-Ribeiro and G. P. Andrade , Universidade Federal de Rural Pernambuco, Recife, Brazil ; and H. R. Pappu , Department of Plant Pathology, University of Georgia, Coastal Plain Experimental Station, Tifton 31793

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Accepted for publication 24 June 2001.

The Brazilian strain of Cowpea aphid-borne mosaic virus (CABMV) is a severe pathogen in peanut and a significant problem when distributing germ plasm to other countries. The virus is seedborne at approximately 0.15% in peanut, depending upon the cultivar, and its detection in seed lots would strengthen quarantine programs. Utilizing 3′ sequence data (GenBank Accession #AF241233), primers were designed from the coat protein region and evaluated by reverse transcription-polymerase chain reaction (RT-PCR). Use of the forward primer 5′-CGCTCAAACCCATTGTAGAA-3′ and reverse primer 5′-TATTGCTTCCCTTGCTCTTTC-3′ yielded a 221-bp product. Extracts of thick seed slices and a sample size of 12 to 25 seed showed no significant advantage of RT-PCR over enzyme-linked immunosorbent assay (ELISA) in tests of large seed lots. However, RT-PCR detected more virus in seed than in the number of infected seedlings normally arising in germination tests. Also, RT-PCR was extremely sensitive and detected 1 infected leaf among 99 healthy leaves. In contrast, ELISA detected only one infected leaf among nine healthy leaves.

The American Phytopathological Society, 2001