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Evaluation of Continuous Application Technology for Delivery of the Biocontrol Agent Pseudomonas putida 06909-rif/nal

April 2001 , Volume 85 , Number  4
Pages  387 - 392

K. Steddom , Texas Agricultural Experiment Station, 2301 Experiment Station Road, Bushland 79012-0010 ; and J. A. Menge , Department of Plant Pathology, University of California, Riverside 92521-0001

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Accepted for publication 13 November 2000.

We evaluated a commercial field fermentor as a means of culturing and delivering a bacterial biocontrol agent, Pseudomonas putida 06909-rif/nal, through irrigation water. There was no evidence of contamination in 12-hour cultures produced by the fermentor. It produced 120 liters of inoculum at 5 × 108 CFU/ml after 12-hour fermentations, allowing two applications per day. Dilutions up to 1:100,000 of inoculum of P. putida 06909-rif/nal produced by the fermentor allowed the biocontrol agent to effectively colonize soil in greenhouse trials. Bacteria produced in 19-hour fermentations colonized soil better than bacteria produced in 12-hour fermentations. Ten repetitive applications of P. putida 06909-rif/nal yielded soil population levels similar to those from a single application at 10-fold greater concentrations. Repetitive applications of lower concentrations may be a cost-effective method for delivering bacterial biocontrol agents to large acreages. This work suggests that the commercial field fermentor would be a viable instrument for delivering bacterial biocontrol agents for commercial-scale field applications.

© 2001 The American Phytopathological Society