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Particle Lengths of Whitefly-Transmitted Criniviruses

July 2000 , Volume 84 , Number  7
Pages  803 - 805

H.-Y. Liu , G. C. Wisler , and J. E. Duffus , United States Department of Agriculture-Agricultural Research Service, 1636 East Alisal Street, Salinas, CA 93905

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Accepted for publication 14 April 2000.

An improved method for particle length measurement was used for six members of the genus Crinivirus. Particle measurements were conducted with a CCD-72S solid state camera, which was interfaced with a Zeiss EM 109 electron microscope, and analyzed using the analysis 2.1 Image Analysis Software. In comparisons of specimen preparation methods, the leaf dip method is more representative and reproducible than the antibody capture method or preparation from purified virions. Particle length (nm) ranges of whitefly-transmitted criniviruses are: Abutilon yellows virus (AYV), 800 to 850; Cucurbit yellow stunting disorder virus (CYSDV), 750 to 800; Lettuce chlorosis virus (LCV), 800 to 850; Lettuce infectious yellows virus (LIYV), 700 to 750; Tomato chlorosis virus (ToCV), 800 to 850; and Tomato infectious chlorosis virus (TICV), 850 to 900.

Additional keywords: Closteroviridae, histogram

The American Phytopathological Society, 2000