Germination of Cephalosporium gramineum conidia in soil was up to twofold greater at -0.064 MPa than at -0.037 and -0.007 MPa when incubated at 5°C for 2 days. Soil pH from 4.7 to 7.5 did not have a significant influence on germination of conidia and the interaction between soil pH and matric potential on germination was not significant. Soil fungistasis, which was previously observed for conidia of C. gramineum, was not observed in these studies. Germination of conidia on mineral salts agar containing phosphate buffer was significantly less at pH 4.5 than at 5.5, 6.5, or 7.5 at 5°C in one of two experiments; however, pH had no influence on germination at 10 or 20°C in two experiments. Although Cephalosporium stripe is more severe under conditions of high soil moisture and low soil pH, increased germination of conidia in response to these factors does not explain the observed increase in disease.