University of Florida, Gulf Coast Research and Education Center, 5007 60 St. E., Bradenton 34203-9324
University of Florida, Plant Pathology Department, 1453 Fifield Hall, Gainesville 32611-0680
University of Florida, Central Florida-REC, 5336 University Ave., Leesburg 34748-8232
University of Florida, Ft. Lauderdale-REC, 3205 S.W. College Ave., Ft. Lauderdale 33314-7700
University of Florida, Gulf Coast Research and Education Center, Bradenton 34203
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Accepted for publication 07 July 1998.
The location of tomato mottle virus (ToMoV) and cabbage leaf curl virus (CabLCV) (Geminiviridae, genus Begomovirus) in the whitefly vector Bemisia tabaci B-biotype (Homoptera: Aleyrodidae) was elucidated using a novel technique incorporating indirect immunofluorescent labeling in freshly dissected whiteflies. Begomoviruses were visualized in the whitefly by indirect-fluorescent-microscopy. Polyclonal and monoclonal primary antibodies were used to successfully detect both ToMoV and CabLCV. Both begomoviruses were located in the anterior region of the midgut and filter-chamber of adult whiteflies, with ToMoV detected in the salivary glands. CabLCV was detected at a greater frequency than ToMoV, with a positive detection of 16% (89 out of 560) for CabLCV and 3% (25 out of 840) for ToMoV. Possible sites involved in geminivirus transport from the gut lumen of whiteflies into the hemocoel were located in the filter-chamber and anterior portion of the midgut. The location of these begomoviruses suggests a possible scenario of virus movement through the whitefly, which is discussed.
© 1998 The American Phytopathological Society