May
1997
, Volume
81
, Number
5
Pages
489
-
491
Authors
G.
Loebenstein
and
F.
Akad
,
Department of Virology, Agricultural Research Organization, Bet Dagan 50250, Israel
;
V.
Filatov
,
G.
Sadvakasova
, and
A.
Manadilova
,
Institute of Molecular Biology and Biochemistry, Almaty, Kazakhstan
; and
H.
Bakelman
,
E.
Teverovsky
,
O.
Lachmann
, and
A.
David
,
Department of Virology, Agricultural Research Organization, Bet Dagan 50250, Israel
Affiliations
Go to article:
RelatedArticle
Accepted for publication 3 February 1997.
Abstract
ABSTRACT
A digoxigenin-labeled cRNA probe of approximately 2,100 bp was more than 2,000 times more sensitive in detecting potato leafroll virus (PLRV) in leaf extracts of Datura stramonium, Physalis floridana, and potatoes than enzyme-linked immunosorbent assay (ELISA). The limit of detecting PLRV with the probe was 1 pg/ml compared with 2 ng/ml by ELISA. The probe detected PLRV easily in dormant tuber tissues at dilutions of up to 1:100. There was no background reaction with healthy extracts. No reactions were observed between the probe and potato X potexvirus or potato Y potyvirus.
JnArticleKeywords
Additional keywords:
fluorescent-labeled probe
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ArticleCopyright
© 1997 The American Phytopathological Society
