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A Sensitive Assay for Rapid Detection and Quantification of Aphanomyces euteiches in Soil

October 2014 , Volume 104 , Number  10
Pages  1,138 - 1,147

Christophe Gangneux, Marc-Antoine Cannesan, Mélanie Bressan, Lisa Castel, Anne Moussart, Maïté Vicré-Gibouin, Azeddine Driouich, Isabelle Trinsoutrot-Gattin, and Karine Laval

First, second, third, fourth, eighth, and ninth authors: Unité Agri'Terr, Esitpa, 3 rue du Tronquet, CS40118, F-76134 Mont-Saint-Aignan, France; second, sixth, and seventh authors: Laboratoire Glycobiologie et Matrice Extracellulaire Végétale, EA 4358, Université de Rouen, F-76821 Mont Saint Aignan, France; and fifth author: INRA, UMR1349 IGEPP, F-35653 Le Rheu, France, Union Nationale Interprofessionnelle des Plantes Riches en Protéines (UNIP), 11 rue de Monceau–CS60003–F-75378 Paris.

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Accepted for publication 31 March 2014.

Aphanomyces euteiches is a widespread oomycete pathogen causing root rot in a wide range of leguminous crops. Losses can reach up to 100% for pea culture and there is currently no registered pesticide for its control. Crop management remains the most efficient tool to control root rot, and avoidance of infested soil seems to be the optimal solution. A test was developed to identify fields suitable for pea crops, consisting of the determination of the inoculum potential of soil using baiting plants. A new rapid, specific, and sensitive molecular method is described allowing the quantification of less than 10 oospores per gram of soil. This challenge is achieved by a real-time polymerase chain reaction procedure targeting internal transcribed spacer 1 from the ribosomal DNA operons. A preliminary study based on typical soils from northwestern France demonstrated that the A. euteiches oospore density in soil is related to the inoculum potential. Furthermore, this method has proved sensitive enough to accurately study the influence of biotic factors that may govern the actual emergence of root rot.

© 2014 The American Phytopathological Society