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Biological Control of Wheat Root Diseases by the CLP-Producing Strain Pseudomonas fluorescens HC1-07

March 2014 , Volume 104 , Number  3
Pages  248 - 256

Ming-Ming Yang, Shan-Shan Wen, Dmitri V. Mavrodi, Olga V. Mavrodi, Diter von Wettstein, Linda S. Thomashow, Jian-Hua Guo, and David M. Weller

First and seventh authors: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Engineering Center of Bioresource Pesticide in Jiangsu Province, Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Agriculture, Nanjing, 210095, China; first and fourth authors: Department of Plant Pathology, Washington State University, Pullman 99164-6430; second and fifth authors: Department of Crop and Soil Sciences, Washington State University, Pullman 99164-6420; third author: Department of Biological Sciences, The University of Southern Mississippi, Hattiesburg 39406; and sixth and eighth authors: United States Department of Agriculture–Agricultural Research Service, Root Disease and Biological Control Research Unit, Pullman, WA 99164-6420.

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Accepted for publication 14 October 2013.

Pseudomonas fluorescens HC1-07, previously isolated from the phyllosphere of wheat grown in Hebei province, China, suppresses the soilborne disease of wheat take-all, caused by Gaeumannomyces graminis var. tritici. We report here that strain HC1-07 also suppresses Rhizoctonia root rot of wheat caused by Rhizoctonia solani AG-8. Strain HC1-07 produced a cyclic lipopeptide (CLP) with a molecular weight of 1,126.42 based on analysis by electrospray ionization mass spectrometry. Extracted CLP inhibited the growth of G. graminis var. tritici and R. solani in vitro. To determine the role of this CLP in biological control, plasposon mutagenesis was used to generate two nonproducing mutants, HC1-07viscB and HC1-07prtR2. Analysis of regions flanking plasposon insertions in HC1-07prtR2 and HC1-07viscB revealed that the inactivated genes were similar to prtR and viscB, respectively, of the well-described biocontrol strain P. fluorescens SBW25 that produces the CLP viscosin. Both genes in HC1-07 were required for the production of the viscosin-like CLP. The two mutants were less inhibitory to G. graminis var. tritici and R. solani in vitro and reduced in ability to suppress take-all. HC1-07viscB but not HC-07prtR2 was reduced in ability to suppress Rhizoctonia root rot. In addition to CLP production, prtR also played a role in protease production.

Additional keyword: biosurfactant.

This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 2014.