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Potential of DNA Barcoding for Detecting Quarantine Fungi

November 2013 , Volume 103 , Number  11
Pages  1,103 - 1,107

Ruifang Gao and Guiming Zhang

First author: College of Agronomy and Biotechnology, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing 100193, P.R. China; and first and second authors: Animal and Plant Inspection and Quarantine Technology Center, Shenzhen Entry-Exit Inspection and Quarantine Bureau, No. 1011 Fuqiang Road, Shenzhen 518045, P.R. China, and Shenzhen Key Laboratory for Research and Development on Detection Technology of Alien Pests, Shenzhen Academy of Inspection and Quarantine, No. 1011 Fuqiang Road, Shenzhen 518045, P.R. China.

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Accepted for publication 20 May 2013.

The detection of live quarantine pathogenic fungi plays an important role in guaranteeing regional biological safety. DNA barcoding, an emerging species identification technology, holds promise for the reliable, quick, and accurate detection of quarantine fungi. International standards for phytosanitary guidelines are urgently needed. The varieties of quarantine fungi listed for seven countries/regions, the currently applied detection methods, and the status of DNA barcoding for detecting quarantine fungi are summarized in this study. Two approaches have been proposed to apply DNA barcoding to fungal quarantine procedures: (i) to verify the reliability of known internal transcribed spacer (ITS)/cytochrome c oxidase subunit I (COI) data for use as barcodes, and (ii) to determine other barcodes for species that cannot be identified by ITS/COI. As a unique, standardizable, and universal species identification tool, DNA barcoding offers great potential for integrating detection methods used in various countries/regions and establishing international detection standards based on accepted DNA barcodes. Through international collaboration, interstate disputes can be eased and many problems related to routine quarantine detection methods can be solved for global trade.

© 2013 The American Phytopathological Society