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Biological and Molecular Characterization of a U.S. Isolate of Hosta virus X

December 2012 , Volume 102 , Number  12
Pages  1,176 - 1,181

Carola M. De La Torre, Feng Qu, Margaret G. Redinbaugh, and Dennis J. Lewandowski

First and fourth authors: Department of Plant Pathology, The Ohio State University, Columbus 43210; second author: Department of Plant Pathology, Ohio Agricultural Research and Development Center, Wooster 44691; and third author: U.S. Department of Agriculture-Agricultural Research Service, Corn and Soybean Research, Wooster, OH 44691.

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Accepted for publication 3 July 2012.

Hosta virus X (HVX) is rapidly becoming a serious pathogen of commercially important hosta plants worldwide. We report here biological and molecular characterization of a U.S. isolate of HVX, HVX-37. HVX-37 infectivity was tested in 21 hosta cultivars over three growth seasons, and three types of responses were defined based upon the ability of the virus to cause local and/or systemic infections. Four cultivars resistant to systemic HVX infection were identified. The full-length sequence of the HVX-37 genome was determined, the first complete sequence of a U.S. HVX isolate. Comparison with the previously sequenced HVX-Korea (Kr) genome revealed a high level of sequence similarity, as well as some differences. Notably, a 105-nucleotide long, near-perfect direct repeat in the Kr isolate is absent in HVX-37. The accuracy of the HVX-37 genome sequence was confirmed by infectivity of in vitro transcripts synthesized from a full-length HVX-37 cDNA on Nicotiana benthamiana and hosta plants.

© 2012 The American Phytopathological Society