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Pathogenic and Genetic Diversity of Xanthomonas translucens pv. undulosa in North Dakota

April 2012 , Volume 102 , Number  4
Pages  390 - 402

Tika B. Adhikari, Suraj Gurung, Jana M. Hansen, and J. Michael Bonman

First, second, and third authors: Department of Plant Pathology, North Dakota State University, Department 7660, P.O. Box 6050, Fargo 58108; and fourth author: United States Department of Agriculture–Agricultural Research Service, Small Grains and Potato Germplasm Research Unit, Aberdeen, ID 83210.

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Accepted for publication 21 December 2011.

Bacterial leaf streak (BLS), caused by Xanthomonas translucens pv. undulosa, has become more prevalent recently in North Dakota and neighboring states. From five locations in North Dakota, 226 strains of X. translucens pv. undulosa were collected and evaluated for pathogenicity and then selected strains were inoculated on a set of 12 wheat cultivars and other cereal hosts. The genetic diversity of all strains was determined using repetitive sequence-based polymerase chain reaction (rep-PCR) and insertion sequence-based (IS)-PCR. Bacterial strains were pathogenic on wheat and barley but symptom severity was greatest on wheat. Strains varied greatly in aggressiveness, and wheat cultivars also showed differential responses to several strains. The 16S ribosomal DNA sequences of the strains were identical, and distinct from those of the other Xanthomonas pathovars. Combined rep-PCR and IS-PCR data produced 213 haplotypes. Similar haplotypes were detected in more than one location. Although diversity was greatest (≈92%) among individuals within a location, statistically significant (P ≤ 0.001 or 0.05) genetic differentiation among locations was estimated, indicating geographic differentiation between pathogen populations. The results of this study provide information on the pathogen diversity in North Dakota, which will be useful to better identify and characterize resistant germplasm.

Additional keywords: linkage disequilibrium, resistance, Triticum aestivum, virulence.

© 2012 The American Phytopathological Society