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Association Mapping of Quantitative Resistance to Phaeosphaeria nodorum in Spring Wheat Landraces from the USDA National Small Grains Collection

November 2011 , Volume 101 , Number  11
Pages  1,301 - 1,310

Tika B. Adhikari, Eric W. Jackson, Suraj Gurung, Jana M. Hansen, and J. Michael Bonman

First, third, and fourth authors: Department of Plant Pathology, North Dakota State University, Department 7660, P.O. Box 6050, Fargo, ND 58108; second and fifth authors: United States Department of Agriculture–Agricultural Research Service, Small Grains and Potato Germplasm Research Unit, Aberdeen, ID 83210.

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Accepted for publication 10 June 2011.

Stagonospora nodorum blotch (SNB), caused by Phaeosphaeria nodorum, is a destructive disease of wheat (Triticum aestivum) found throughout the United States. Host resistance is the only economically feasible option for managing the disease; however, few SNB-resistant wheat cultivars are known to exist. In this study, we report findings from an association mapping (AM) of resistance to P. nodorum in 567 spring wheat landraces of diverse geographic origin. The accessions were evaluated for seedling resistance to P. nodorum in a greenhouse. Phenotypic data and 625 polymorphic diversity array technology (DArT) markers have been used for linkage disequilibrium (LD) and association analyses. The results showed that seven DArT markers on five chromosomes (2D, 3B, 5B, 6A, and 7A) were significantly associated with resistance to P. nodorum. Genetic regions on 2D, 3B, and 5B correspond to previously mapped quantitative trait loci (QTL) conferring resistance to P. nodorum whereas the remaining QTL appeared to be novel. These results demonstrate that the use of AM is an effective method for identifying new genomic regions associated with resistance to P. nodorum in spring wheat landraces. Additionally, the novel resistance found in this study could be useful in wheat breeding aimed at controlling SNB.

© 2011 The American Phytopathological Society