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Molecular Mapping of the New Blast Resistance Genes Pi47 and Pi48 in the Durably Resistant Local Rice Cultivar Xiangzi 3150

May 2011 , Volume 101 , Number  5
Pages  620 - 626

Hongmei Huang, Ling Huang, Guangping Feng, Suhua Wang, Yue Wang, Jinling Liu, Nan Jiang, Weiting Yan, Lingchao Xu, Pingyong Sun, Zhiqiang Li, Sujun Pan, Xionglun Liu, Yinghui Xiao, Erming Liu, Liangying Dai, and Guo-Liang Wang

First to seventeenth authors: Hunan Provincial Key Laboratory of Crop Gene Engineering, Hunan Agricultural University, Changsha 410128, China; and seventeenth author: Department of Plant Pathology, The Ohio State University, Columbus, Ohio 43210. First and second authors contributed equally to this project.

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Accepted for publication 3 December 2010.

The indica rice cultivar Xiangzi 3150 (XZ3150) confers a high level of resistance to 95% of the isolates of Magnaporthe oryzae (the agent of rice blast disease) collected in Hunan Province, China. To identify the resistance (R) gene(s) controlling the high level of resistance in this cultivar, we developed 286 F9 recombinant inbred lines (RILs) from a cross between XZ3150 and the highly susceptible cultivar CO39. Inoculation of the RILs and an F2 population from a cross between the two cultivars with the avirulent isolate 193-1-1 in the growth chamber indicated the presence of two dominant R genes in XZ3150. A linkage map with 134 polymorphic simple sequence repeat and single feature polymorphism markers was constructed with the genotype data of the 286 RILs. Composite interval mapping (CIM) using the results of 193-1-1 inoculation showed that two major R genes, designated Pi47 and Pi48, were located between RM206 and RM224 on chromosome 11, and between RM5364 and RM7102 on chromosome 12, respectively. Interestingly, the CIM analysis of the four resistant components of the RILs to the field blast population revealed that Pi47 and Pi48 were also the major genetic factors responsible for the field resistance in XZ3150. The DNA markers linked to the new R genes identified in this study should be useful for further fine mapping, gene cloning, and marker-aided breeding of blast-resistant rice cultivars.

Additional keywords: Oryza sativa.

© 2011 The American Phytopathological Society