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Multigenic System Controlling Viral Systemic Infection Determined by the Interactions Between Cucumber mosaic virus Genes and Quantitative Trait Loci of Soybean Cultivars

May 2011 , Volume 101 , Number  5
Pages  575 - 582

Shizen Ohnishi, Issei Echizenya, Eri Yoshimoto, Kim Boumin, Tsuyoshi Inukai, and Chikara Masuta

Graduate School of Agriculture, Hokkaido University, 060-8589 Sapporo, Japan.

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Accepted for publication 10 December 2010.

Soybean ‘Harosoy’ is resistant to Cucumber mosaic virus soybean strain C (CMV-SC) and susceptible to CMV-S strain D (CMV-SD). Using enzyme-linked immunosorbent assay and Northern hybridization, we characterized the Harosoy resistance and found that CMV-SC did not spread systemically but was restricted to the inoculated leaves in Harosoy. Harosoy resistance was not controlled by either a dominant or recessive single gene. To dissect this system controlling long-distance movement of CMV in soybean, we constructed infectious cDNA clones of CMV-SC and CMV-SD. Using these constructs and the chimeric RNAs, we demonstrated that two viral components were required for systemic infection by the virus. The region including the entire 2b gene and the 5′ region of RNA3 (mainly the 5′ untranslated region) together were required. By quantitative trait locus (QTL) analysis using an F2 population and the F3 families derived from Harosoy and susceptible ‘Nemashirazu’, we also showed that at least three QTLs affected systemic infection of CMV in soybean. Our study on Harosoy resistance to CMV-SC revealed an interesting mechanism, in which multiple host and viral genes coordinately controlled viral systemic infection.

© 2011 The American Phytopathological Society