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Quantitative Trait Loci in the Ogle/TAM O-301 Oat Mapping Population Controlling Resistance to Puccinia coronata in the Field

May 2010 , Volume 100 , Number  5
Pages  484 - 492

E. W. Jackson, D. E. Obert, J. B. Avant, S. A. Harrison, J. Chong, M. L. Carson, and J. M. Bonman

First, second, third, and seventh authors: United States Department of Agriculture–Agricultural Research Service (USDA-ARS), Small Grains and Potato Germplasm Research Unit, 1691 S. 2700 W., Aberdeen, ID 83210; fourth author: Louisiana State University (LSU), Wheat, Oat & Coastal Plants Breeding, LSU School of Plant, Environmental and Soil Sciences, 104 Sturgis Hall, Baton Rouge 70803-2110; fifth author: Agriculture and Agri-Food Canada, 195 Dafoe Rd., Winnipeg, Manitoba R3T 2M9, Canada; and sixth author: USDA-ARS, Cereal Disease Laboratory, 1551 Lindig Avenue, University of Minnesota, St. Paul 55108.

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Accepted for publication 24 December 2009.

Crown rust is the most damaging disease of cultivated oat (Avena sativa) and genetic resistance is the primary means of controlling the disease. Quantitative trait loci (QTL) with major and minor effects have been identified in Ogle1040 and TAM O-301 (most notably, Pc58 and PcNQMG/LGCG from TAM O-301 and OT-27 from Ogle1040) through single-isolate greenhouse and field tests. To map loci and determine the effectiveness of previously identified QTL against naturally occurring pathogen populations in highly disease-conducive environments, the Ogle/TAM O-301 (OT) recombinant inbred line (RIL) population was grown in Texas and Louisiana over 2 years and in Manitoba, Canada. The genetic region characterized by the Pc58 resistance gene complex, particularly Pc58a, accounted for most of the diseased leaf area (DLA) and infection type (IT) variance in all five experiments. Additionally, the genetic region characterized by PcNQMG/LGCG accounted for a portion of the IT variance in three experiments. Although no QTL was detected on OT-27 in this study, all the markers on this linkage group were associated (P < 0.0001) with reducing both IT and DLA using single-marker analysis. Screening with 25 Puccinia coronata isolates from six different states indicated that Pc58abc and Pc58a were highly effective, while characterization using F2 populations derived from OT RILs containing the two main genetic regions responsible for crown rust resistance in TAM O-301 (Pc58 and PcNQMG/LGCG) and a minor QTL in Ogle (OT-27) indicated that Pc58a, in combination with a locus in Ogle1040, provided high levels of resistance to natural races in Texas. This study provides new information and key loci in OT mapping population and may be useful for effective control of crown rust in North America.

The American Phytopathological Society, 2010