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Vegetative Compatibility Groups in Colletotrichum coccodes Subpopulations from Australia and Genetic Links with Subpopulations from Europe/Israel and North America

March 2010 , Volume 100 , Number  3
Pages  271 - 278

B. Ben-Daniel, D. Bar-Zvi, D. Johnson, R. Harding, M. Hazanovsky, and L. Tsror (Lahkim)

First, fifth, and sixth authors: Department of Plant Pathology, Agricultural Research Organization, Gilat Research Center, M. P. Negev 85280, Israel; first and second authors: Department of Life Sciences, Ben-Gurion University, Be'er-Sheva, Israel; third author: Department of Plant Pathology, Washington State University, Pullman 99164-6430; and fourth author: Department of Plant Pathology, South Australian Research and Development Institute, GPO Box 397, Adelaide, SA 5001, Australia.

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Accepted for publication 15 November 2009.

Vegetative compatibility of 94 isolates of Colletotrichum coccodes from Australia originating from potato, soil, and a weed (Solanum esuriale) was tested using nitrate-nonutilizing (nit) mutants. Isolates distributed to six vegetative compatibility groups (VCGs), five of them multimember (24.5, 23.4, 13.8, 12.8, and 7.5% distribution) and only one composed of two isolates (2.1%); 15.9% of them were not assigned to any of the VCGs. Aggressiveness of 51 isolates representing all six VCGs was tested by mature green tomato bioassay: isolates assigned to AUS-VCG-4 were the most aggressive and those in AUS-VCG-3 the least (P < 0.05). Isolates from warmer climates and lower latitudes were more aggressive (P < 0.05). In addition, we report for the first time complementations between isolates from Australia (AUS); North America (NA); and Israel, The Netherlands, Scotland, France, Germany (EU/I). Isolates assigned to AUS-VCG-4 anastomosed with isolates assigned to EU/I-VCG-7 and NA-VCG-5 (which also anastomosed with each other). Isolates assigned to EU/I-VCG-6 anastomosed with isolates assigned to NA-VCG-2 and isolates assigned to AUS-VCG-2 anastomosed with isolates assigned to EU/I-VCG-2. The linkage between subpopulations could result from the limited exchange of seed tubers among continents, or could be due to, for instance, gene flow, selection, or a limited number of polymorphic vegetative incompatibility genes.

Additional keywords:aggressiveness, anastomosis, black dot.

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