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Mapping Net Form Net Blotch and Septoria Speckled Leaf Blotch Resistance Loci in Barley

January 2010 , Volume 100 , Number  1
Pages  80 - 84

S. St. Pierre, C. Gustus, B. Steffenson, R. Dill-Macky, and K. P. Smith

First, second, and fifth authors: Department of Agronomy and Plant Genetics, 411 Borlaug Hall, and third and fourth authors: Department of Plant Pathology, 495 Borlaug Hall, University of Minnesota, 1991 Upper Buford Circle, St. Paul 55108.

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Accepted for publication 4 September 2009.

Septoria speckled leaf blotch (SSLB), caused by Septoria passerinii Sacc., and net form net blotch (NB), caused by Pyrenophora teres f. teres Drechsler, are fungal diseases that decrease the yields of barley in the Upper Midwest. An effective way to manage these diseases is to plant resistant cultivars. To characterize the genetics of resistance to both pathogens, two advanced barley breeding lines, one resistant to NB (M120) and another resistant to SSLB (Sep2-72), were crossed, creating a population of 115 recombinant inbred lines. The two parents and the population were evaluated in three greenhouse seedling assays for each pathogen and for simple-sequence repeat and diversity arrays technology markers. Composite interval mapping revealed two major quantitative trait loci (QTL) associated with NB on chromosome 6H, located in bins 2 and 6. The QTL located in bin 6 explained 19 to 48% of the phenotypic variation and the QTL located in bin 2 explained 25 to 44% of the phenotypic variation. A new locus for resistance to SSLB, Rsp4, was identified on chromosome 6H, located in bins 3 to 4. Mapping these genes in elite breeding germplasm will accelerate the development and utilization of marker-assisted selection to enhance resistance to these diseases.

Additional keywords:disease resistance, Hordeum vulgare.

© 2010 The American Phytopathological Society