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Identification of Race 1 of Fusarium oxysporum f. sp. lactucae on Lettuce by Inter-Retrotransposon Sequence-Characterized Amplified Region Technique

August 2007 , Volume 97 , Number  8
Pages  987 - 996

Matias Pasquali , Flavia Dematheis , Maria Lodovica Gullino , and Angelo Garibaldi

Centre of Competence for the Innovation in the Agro-Environmental Sector (AGROINNOVA), Università degli Studi di Torino, via L. da Vinci 44, I-10095 Grugliasco, Torino, Italy.

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Accepted for publication 26 February 2007.

Fusarium wilt of lettuce, caused worldwide by Fusarium oxysporum f. sp. lactucae, is an emerging seed-transmitted disease on Lactuca sativa. In order to develop a molecular diagnostic tool for identifying race 1 (VCG0300) of the pathogen on vegetable samples, an effective technique is presented. Inter-retrotransposon amplified polymorphism polymerase chain reaction (PCR), a technique based on the amplification of genomic regions between long terminal repeats, was applied. It was shown to be useful for grouping F. oxysporum f. sp. lactucae race 1 isolates. Inter-retrotransposon sequence-characterized amplified regions (IR-SCAR) was used to develop a specific set of PCR primers to be utilized for differentiating F. oxysporum f. sp. lactucae isolates from other F. oxysporum isolates. The specific primers were able to uniquely amplify fungal genomic DNA from race 1 isolates obtained in Italy, Portugal, the United States, Japan, and Taiwan. The primers also were specific to pathogen DNA obtained from artificially infected lettuce seed and naturally and artificially infected plants.

Additional keywords: diagnostics, IRAP.

© 2007 The American Phytopathological Society