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Sampling for the Incidence of Aphid-Transmitted Viruses in Snap Bean

December 2005 , Volume 95 , Number  12
Pages  1,405 - 1,411

Denis A. Shah , Helene R. Dillard , and Brian A. Nault

First and second authors: Departments of Plant Pathology; and third author: Entomology, New York State Agricultural Experiment Station, 630 W. North St., Geneva 14456

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Accepted for publication 29 July 2005.

Data collected in 2002 and 2003 on Alfalfa mosaic virus and Cucumber mosaic virus incidences of infection in commercial snap bean fields in New York State were used to develop relationships between disease incidence (plow) and sample size while accounting for the inherent spatial aggregation of infected plants observed with these two viruses. For a plan consisting of 300 sampled plants (N = 60 quadrats, n = 5 plants per quadrat), estimating plow from the incidence of positive groups (phigh; testing of N = 60 grouped samples) provides the same precision in plow as testing 200 plants individually, up to about plow = 0.2. Above that, the confidence interval width for plow obtained via group testing becomes markedly larger than the width obtained by testing individual plants. Our results suggest using group testing until phigh is in the range [0.35, 0.59], which corresponds to plow in [0.1, 0.2]. Results indicate that group testing can be more economical than the testing of individual plants without loss of precision, at lower incidences of infection. The approach presented provides a general framework for sampling and the estimation of incidence of other aphid-transmitted viruses in snap bean.

Additional keywords: cluster sampling , hierarchical sampling , virus epidemiology .

© 2005 The American Phytopathological Society