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A Cluster of Major Specific Resistance Genes to Leptosphaeria maculans in Brassica napus

June 2004 , Volume 94 , Number  6
Pages  578 - 583

R. Delourme , M. L. Pilet-Nayel , M. Archipiano , R. Horvais , X. Tanguy , T. Rouxel , H. Brun , M. Renard , and M. H. Balesdent

First, second, third, fourth, fifth, and eighth authors: INRA, UMR APBV, BP 35327, 35653 Le Rheu Cedex, France; sixth and ninth authors: INRA, UMR PMDV, Route de Saint-Cyr, 78026 Versailles Cedex, France; and seventh author: INRA, UMR BiO3P, BP 35327, 35653 Le Rheu Cedex, France

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Accepted for publication 26 January 2004.

Two types of genetic resistance to Leptosphaeria maculans usually are distinguished in Brassica napus: qualitative, total resistance expressed at the seedling stage and quantitative, partial resistance expressed at the adult plant stage. The latter is under the control of many genetic factors that have been mapped through quantitative trait loci (QTL) studies using ‘Darmor’ resistance. The former usually is ascribed to race-specific resistance controlled by single resistance to L. maculans (Rlm) genes. Three B. napus-originating specific Rlm genes (Rlm1, Rlm2, and Rlm4) previously were characterized. Here, we report on the genetic identification of two novel resistance genes, Rlm3 and Rlm7, corresponding to the avirulence genes AvrLm3 and AvrLm7. The identification of a novel L. maculans- B. napus specific interaction allowed the detection of another putative new specific resistance gene, Rlm9. The resistance genes were mapped in two genomic regions on LG10 and LG16 linkage groups. A cluster of five resistance genes (Rlm1, Rlm3, Rlm4, Rlm7, and Rlm9) was strongly suggested on LG10. The relation between all these specific resistance genes and their potential role in adult-plant field resistance is discussed. These two Rlm-carrying regions do not correspond to major QTL for Darmor quantitative resistance.

Additional keywords: blackleg, Phoma lingam.

The American Phytopathological Society, 2004