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Generation and Molecular Mapping of a Sequence Characterized Amplified Region Marker Linked with the Bct Gene for Resistance to Beet curly top virus in Common Bean

April 2004 , Volume 94 , Number  4
Pages  320 - 325

Richard C. Larsen and Phillip N. Miklas

U.S. Department of Agriculture-Agriculture Research Service, Vegetable and Forage Crop Research Unit, 24106 North Bunn Road, Prosser, WA 99350

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Accepted for publication 9 December 2003.

A random amplified polymorphic DNA (RAPD) marker directly linked (0.0 cM) with a resistance gene was identified in a snap bean recombinant inbred population (Moncayo × Primo) consisting of 94 F5:7 recombinant inbred lines that had uniform segregation for disease reaction to Beet curly top virus (BCTV) across three field locations. Resistance was conditioned by a single dominant allele tentatively designated Bct. Seven hundred and fifty decamer primers were screened to obtain the linked RAPD marker that was then converted to a sequence characterized amplified region (SCAR) marker SAS8.1550. The SCAR mapped within a cluster of resistance genes on linkage group B7 of the core map. A survey of 103 BCTV-resistant and -susceptible snap and dry bean genotypes was conducted using SAS8.1550. Results showed that the SCAR would be highly useful for marker-assisted selection of Bct in snap and dry bean originating from the Andean gene pool. Marker-assisted selection for Bct will expedite the development of BCTV-resistant cultivars and minimize the need for cumbersome pathogen tests.

Additional keywords: begomovirus, bulked-segregant analysis, polymerase chain reaction-based DNA marker.

The American Phytopathological Society, 2004