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Induction of Chitinase, β-1,3-Glucanase, and Phenylalanine Ammonia Lyase in Peach Fruit by UV-C Treatment

March 2003 , Volume 93 , Number  3
Pages  349 - 355

Ahmed El Ghaouth , Charles L. Wilson , and Ann M. Callahan

First author: Universite De Nouakchott, Faculte des Sciences et Techniques, Nouakchott, Mauritania, B.P. 5026; and first, second, and third authors: U.S. Department of Agriculture-Agricultural Research Service, Appalachian Fruit Research Station, 45 Wiltshire Road, Kearneysville, WV 25430

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Accepted for publication 1 October 2002.

Treatment of peach fruit with UV-C light caused a rapid induction of chitinase, β-1,3-glucanase, and phenylalanine ammonia lyase (PAL) activities starting 6 h after treatment and reaching maximum levels at 96 h after treatment. By 96 h after UV-C treatment, chitinase, β-1,3-glucanase, and PAL activities in UV-C-treated fruit were over twofold above the levels observed for the control. In nontreated control fruit, no apparent increase in chitinase and β-1,3-glucanase activities was detected but a minor increase in PAL activity was seen. The transient increase in chitinase, β-1,3-glucanase, and PAL activities in UV-C-treated fruit was preceded by a gradual activation of the corresponding genes. UV-C-treated fruit showed an increase in accumulation of β-1,3-glucanase and chitinase mRNAs at 3 h after treatment, which peaked approximately 96 h posttreatment. A similar induction kinetic pattern was observed for PAL mRNA in response to UV-C treatment, except the induction started 6 h after UV-C treatment. These results show that the response of peach fruit to elicitor treatment is similar to that seen in other plant-elicitors interactions and suggests the involvement of peach biochemical defense responses in UV-C-mediated disease resistance.

Additional keywords: antagonistic yeast, apple, Botrytis cinerea, gray mold.

The American Phytopathological Society, 2003