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Genetic Diversity and Pathogenic Variability Among Isolates of Colletotrichum Species from Strawberry

February 2003 , Volume 93 , Number  2
Pages  219 - 228

Béatrice Denoyes-Rothan , Guy Guérin , Christophe Délye , Barbara Smith , Dror Minz , Marcel Maymon , and Stanley Freeman

First and second authors: UREFV, INRA, BP 81, 33883, Villenave d'Ornon Cedex, France; third author: Laboratoire de Malherbologie et Agronomie, INRA, BV 1540, 21034, Dijon Cedex, France; fourth author: U.S. Department of Agriculture-Agriculture Research Services, Poplarville, MS 39470; fifth author: Institute of Soil, Water and Environmental Sciences, ARO, The Volcani Center, P.O. Box 6, Bet Dagan 50250; and sixth and seventh authors: Department of Plant Pathology, ARO, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel

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Accepted for publication 25 September 2002.

Ninety-five isolates of Colletotrichum including 81 isolates of C. acutatum (62 from strawberry) and 14 isolates of C. gloeosporioides (13 from strawberry) were characterized by various molecular methods and pathogenicity tests. Results based on random amplified polymorphic DNA (RAPD) polymorphism and internal transcribed spacer (ITS) 2 sequence data provided clear genetic evidence of two subgroups in C. acutatum. The first subgroup, characterized as CA-clonal, included only isolates from strawberry and exhibited identical RAPD patterns and nearly identical ITS2 sequence analysis. A larger genetic group, CA-variable, included isolates from various hosts and exhibited variable RAPD patterns and divergent ITS2 sequence analysis. Within the C. acutatum population isolated from strawberry, the CA-clonal group is prevalent in Europe (54 isolates of 62). A subset of European C. acutatum isolates isolated from strawberry and representing the CA-clonal and CA-variable groups was assigned to two pathogenicity groups. No correlation could be drawn between genetic and pathogenicity groups. On the basis of molecular data, it is proposed that the CA-clonal subgroup contains closely related, highly virulent C. acutatum isolates that may have developed host specialization to strawberry. C. gloeosporioides isolates from Europe, which were rarely observed were either slightly or nonpathogenic on strawberry. The absence of correlation between genetic polymorphism and geographical origin in Colletotrichum spp. suggests a worldwide dissemination of isolates, probably through international plant exchanges.

Additional keywords: Glomerella cingulata , internal transcribed spacer, phylogeny, rDNA.

The American Phytopathological Society, 2003