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Origin of Race 3 of Fusarium oxysporum f. sp. lycopersici at a Single Site in California

August 2003 , Volume 93 , Number  8
Pages  1,014 - 1,022

G. Cai , L. Rosewich Gale , R. W. Schneider , H. C. Kistler , R. M. Davis , K. S. Elias , and E. M. Miyao

First, third, and sixth authors: Department of Plant Pathology and Crop Physiology, Louisiana State University Agricultural Center, Baton Rouge 70803; second and fourth authors: U.S. Department of Agriculture, Agricultural Research Service, Cereal Disease Laboratory, and Department of Plant Pathology, University of Minnesota, St. Paul 55108; fifth author: Department of Plant Pathology, University of California, Davis 95616; and seventh author, University of California Cooperative Extension, Woodland 95695

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Accepted for publication 7 April 2003.

Thirty-nine isolates of Fusarium oxysporum were collected from tomato plants displaying wilt symptoms in a field in California 2 years after F. oxysporum f. sp. lycopersici race 3 was first observed at that location. These and other isolates of F. oxysporum f. sp. lycopersici were characterized by pathogenicity, race, and vegetative compatibility group (VCG). Of the 39 California isolates, 22 were in VCG 0030, 11 in VCG 0031, and six in the newly described VCG 0035. Among the isolates in VCG 0030, 13 were race 3, and nine were race 2. Of the isolates in VCG 0031, seven were race 2, one was race 1, and three were nonpathogenic to tomato. All six isolates in VCG 0035 were race 2. Restriction fragment length polymorphisms (RFLPs) and sequencing of the intergenic spacer (IGS) region of rDNA identified five IGS RFLP haplotypes, which coincided with VCGs, among 60 isolates of F. oxysporum from tomato. Five race 3 isolates from California were of the same genomic DNA RFLP haplotype as a race 2 isolate from the same location, and all 13 race 3 isolates clustered together into a subgroup in the neighbor joining tree. Collective evidence suggests that race 3 in California originated from the local race 2 population.

© 2003 The American Phytopathological Society