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A Point Mutation in the Two-Component Histidine Kinase BcOS-1 Gene Confers Dicarboximide Resistance in Field Isolates of Botrytis cinerea

January 2002 , Volume 92 , Number  1
Pages  75 - 80

Michiyo Oshima , Makoto Fujimura , Shinpei Banno , Chigusa Hashimoto , Takayuki Motoyama , Akihiko Ichiishi , and Isamu Yamaguchi

First, second, third, fourth, and sixth authors: Faculty of Life Science, University of Toyo, Itakura, Oura-gun 374-0193, Japan; and fifth and seventh authors: Plant Science Center, RIKEN, 2-1 Hirosawa, Wako 351-0198, Japan

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Accepted for publication 24 September 2001.

Partial DNA fragments of Botrytis cinerea field isolates encoding the putative osmosensor histidine kinase gene (BcOS1) were cloned by polymerase chain reaction amplification and the predicted amino acid sequences were compared between dicarboximide-sensitive and resistant field isolates. The predicted BcOS1p is highly homologous to osmosensor histidine kinase OS1p from Neurospora crassa including the N-terminal six tandem repeats of approximately 90 amino acids. Four dicarboximide-resistant isolates of B. cinerea (Bc-19, Bc-45, Bc-682, and Bc-RKR) contained a single base pair mutation in their BcOS1 gene that resulted in an amino acid substitution in the predicted protein. In these resistant isolates, codon 86 of the second repeat, which encodes an isoleucine residue in sensitive strains, was converted to a codon for serine. The mutation of Botrytis field resistant isolates was located on the second unit of tandem amino acid repeats of BcOS1p, whereas the point mutations of the fifth repeat of OS1p confer resistance to both dicarboximides and phenylpyrroles and also osmotic sensitivity in Neurospora crassa. These results suggest that an amino acid substitution within the second repeat of BcOS1p is responsible for phenotypes of field resistant isolates (resistant to dicarboximides but sensitive to phenylpyrroles, and normal osmotic sensitivity) in B. cinerea.

Additional keywords: fungicide, glycerol, two-component signal transduction.

© 2002 The American Phytopathological Society