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Variation Among and Within Populations of the Parasitic Weed Orobanche crenata from Spain and Israel Revealed by Inter Simple Sequence Repeat Markers

First and fourth authors: Departamento de Mejora y Agronomía CIFA -Alameda del Obispo, Apdo 3092, 14080 Córdoba, Spain; second author: Faculty of Agriculture. Department of Seed Science and Technology, Svetosimunska 25, 10000 Zagreb, Croatia; third author: CSIC-Instituto de Agricultura Sostenible, Apdo. 4084, 14080 Córdoba, Spain; fifth author: Departamento de Genética. ETSIAM-UCO, Apdo 3048, 14080 Córdoba, Spain; sixth author: The Genetic Lab, Newe-Yáar Research Center, ARO, P.O. Box 1021, Ramat-Yishay 30095, Israel; and seventh author: Department of Weed Research, Newe-Yáar Research Center, ARO, P.O. Box 1021, Ramat-Yishay 30095, Israel

The patterns of genetic variation among Orobanche crenata populations from Spain and Israel were studied using radiolabeled inter simple sequence repeat amplification products that were separated in sequencing polyacrylamide gels. The analysis of molecular variance indicated that most of the genetic diversity was attributable to differences among individuals within a population although significant divergences were found between regions. The Jaccard's similarity matrix was analyzed by unweighted pair-group method with arithmetic average and the resultant dendrogram clearly divided six populations by region, with the Spanish populations being more similar to each other than the Israeli populations. These results are consistent with the predominantly allogamous behavior of O. crenata and the extremely efficient dispersal of its seeds.

Additional keywords: homogeneity of molecular variance, Orobanche resistance, population structure.