Link to home

Molecular Identification of a Sexual Interloper: The Pear Pathogen, Venturia pirina, has Sex on Apple

July 2001 , Volume 91 , Number  7
Pages  633 - 641

Christiane Stehmann , Shaun Pennycook , and Kim M. Plummer

First and second authors: HortResearch, Mt. Albert Research Centre, 120 Mt. Albert Rd., Private Bag 92169, Auckland, New Zealand; and third author: The University of Auckland, School of Biological Sciences, Private Bag 92019 New Zealand

Go to article:
Accepted for publication 23 March 2001.

Venturia pirina (the pear scab pathogen) and V. inaequalis (the apple scab pathogen) were detected as ascospores discharged from apple leaf litter in New Zealand (spring 1998). Pseudothecia of both species were located on dead apple leaves; however, only those of V. inaequalis were associated with scab lesions. V. pirina was identified by rDNA sequence analyses, because morphological characters could not distinguish this fungus from V. asperata (a rare saprophyte on apple) and other Venturia spp. pathogenic on rosaceous fruit trees. Species-specific polymerase chain reaction primers designed to the 18S end of the internal transcribed spacer 1 region differentiated Venturia fruit tree pathogens reliably. V. pirina field isolates were pathogenic on pear, but only weak saprophytes on apple. In rare instances, when appressoria of V. pirina appeared to penetrate the cuticle of apple leaves, epidermal cells responded with a localized hypersensitive response (HR). To our knowledge, this is the first report of induction of HR-like events by V. pirina on its nonhost, apple, and also the first record of sexual reproduction of V. pirina on apple. It is assumed that V. pirina pseudothecia formed from saprophytic lesions in senescing apple leaves when active defense mechanisms such as HR were no longer induced.

Additional keywords: disease prediction , molecular species identification , nonhost resistance , phylogenetic analysis .

© 2001 The American Phytopathological Society