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Systemic and Local Responses Associated with UV- and Pathogen-Induced Resistance to Botrytis cinerea in Stored Carrot

September 2000 , Volume 90 , Number  9
Pages  981 - 986

Julien Mercier , Dominique Roussel , Marie-Thérèse Charles , and Joseph Arul

Département des sciences des aliments et de nutrition and Centre de recherche en horticulture, Université Laval, Sainte-Foy, Quebec G1K 7P4, Canada

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Accepted for publication 25 May 2000.

The induction of resistance to Botrytis cinerea in carrot roots by UV radiation, a possible means for controlling storage diseases, was compared with systemic resistance induced by inoculation with the pathogen. UV radiation did not have any systemic effect, and disease resistance was induced only in tissues directly exposed to the radiation. Although UV radiation induced a local accumulation of 6-methoxymellein (6-MM), inoculation with B. cinerea caused 6-MM to accumulate systemically, away from the inoculation site. Because of amounts near or higher than the ED50 (50% effective dose) for inhibiting B. cinerea found in both UV-treated and preinoculated roots at the time of challenge, 6-MM could be involved in both types of resistance. Freshly harvested carrots had a number of constitutive chitinases and β-1,3-glucanases, which were not affected by UV radiation or inoculation. When challenged with B. cinerea, the induction of a 24-kDa chitinase was enhanced in UV-treated and preinoculated roots. Again, UV radiation had only a local effect in priming this chitinase response. Although UV- and pathogen-induced resistance in carrot may involve the same defenses, the responses are probably mediated differently, because UV radiation has an essentially local effect.

Additional keywords: Daucus carota , isocoumarin, phytoalexin, postharvest, ultraviolet.

© 2000 The American Phytopathological Society