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Comparative Analysis of Indices in the Study of Virulence Diversity Between and Within Populations of Puccinia recondita f. sp. tritici in Israel

June 2000 , Volume 90 , Number  6
Pages  601 - 607

J. Manisterski , Z. Eyal , P. Ben-Yehuda , and E. Kosman

All authors: Institute for Cereal Crops Improvement (ICCI), The George S. Wise Faculty for Life Sciences; second author: Department of Plant Sciences, The George S. Wise Faculty for Life Sciences; fourth author: School of Mathematical Sciences, The Raymond and Beverly Sackler Faculty of Exact Sciences, Tel Aviv University, Tel Aviv 69978, Israel

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Accepted for publication 25 January 2000.

Isolates of Puccinia recondita f. sp. tritici (n = 260) obtained from bread, durum, and wild emmer wheat leaf collections throughout Israel during 1993 to 1997 were analyzed for virulence on a set of wheat differentials. The overall frequency of virulence increased on differentials possessing resistance genes Lr1, Lr2a, Lr3, and Lr26 and decreased on Lr17, Lr21, and Lr30. Genes Lr9 and Lr24 were resistant, while Lr18 was susceptible (98% in 1996) to all tested leaf rust isolates and Lr10 and Lr23 were susceptible to more than 78% of the isolates. Diversity between populations (years) was assessed using Kosman's HKB (based on degrees of similarity among distinct phenotypes) and HKDis (based on frequencies of individual virulences) and Nei's and Rogers' distances. The greatest difference occurred between the 1993 and 1994 populations. Phenotypic diversity within each population (year) was analyzed using the Shannon's, Simpson's, and Kosman's indices. The highest diversity within years was recorded in 1994 and significantly increased from 1993 to 1994 for all indices. The variance in the diversity between populations can be only partially explained by differences between corresponding diversities within population. The comparative analysis of diversity between and within populations over the 5 years enabled a detailed study of changes in the pathogen population. The results show that the different measures do not yield the same rank order of diversity.

Additional keywords: leaf rust, Triticum spp.

© 2000 The American Phytopathological Society