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Genetic Diversity Among Banana streak virus Isolates from Australia

August 2000 , Volume 90 , Number  8
Pages  921 - 927

A. D. W. Geering , L. A. McMichael , R. G. Dietzgen , and J. E. Thomas

First, second, and fourth authors: Queensland Horticulture Institute, Department of Primary Industries, 80 Meiers Road, Indooroopilly, Queensland 4068, Australia; and third author: Queensland Agricultural Biotechnology Centre, Department of Primary Industries, Gehrmann Laboratories, University of Queensland, St. Lucia, Queensland 4072, Australia

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Accepted for publication 19 April 2000.

Banana streak virus (BSV) is an important pathogen of bananas and plantains (Musa spp.) throughout the world. We have cloned and sequenced part of the genomes of four isolates of BSV from Australia, designated BSV-RD, BSV-Cav, BSV-Mys, and BSV-GF. These isolates originated from banana cvs. Red Dacca, Williams, Mysore, and Goldfinger, respectively. All clones contained a sequence covering part of open reading frame III and the intergenic region of the badnavirus genome. The sequences were compared with those of other badnaviruses, including BSV-Onne, a previously characterized isolate from Nigeria. The BSV-RD sequence was virtually identical to that of BSV-Onne, differing by only two nucleotides over 1,292 bp. However, BSV-Cav, -Mys, and -GF were divergent in nucleotide sequence. Phylogenetic analyses using conserved sequences in the ribonuclease H domain revealed that all BSV isolates were more closely related to each other than to any other badnavirus. BSV-Cav was most closely related to BSV-Onne, and there was 95.1% identity between the two amino acid sequences. Other relationships between the BSV isolates were less similar, with sequence identities ranging from 66.4 to 78.2%, which is a magnitude comparable to the distance between some of the recognized badnavirus species. Immunocapture-polymerase chain reaction assays have been developed, allowing specific detection and differentiation of the four isolates of BSV.

Additional keyword: Banana streak disease.

© 2000 The American Phytopathological Society