Link to home

Rapid Detection of the Fusarium oxysporum Lineage Containing the Canary Island Date Palm Wilt Pathogen

May 1999 , Volume 89 , Number  5
Pages  407 - 413

T. R. Plyler , G. W. Simone , D. Fernandez , and H. C. Kistler

First, second, and fourth authors: Department of Plant Pathology, University of Florida, Gainesville 32611; and third author: Laboratoire de Phytopathologie, ORSTOM, B.P. 5045, 34032 Montpellier Cedex 1, France

Go to article:
Accepted for publication 13 January 1999.

Fusarium oxysporum f. sp. canariensis causes Fusarium wilt disease on the Canary Island date palm (Phoenix canariensis). To facilitate disease management, a polymerase chain reaction diagnostic method has been developed to rapidly detect the pathogen. A partial genomic library of F. oxysporum f. sp. canariensis isolate 95-913 was used to identify a DNA sequence diagnostic for a lineage containing all tested isolates of F. oxysporum f. sp. canariensis. Two oligonucleotide primers were designed and used to amplify a 567-bp fragment with F. oxysporum f. sp. canariensis DNAs. DNA from 61 outgroup isolates did not amplify using these primers. Once the primers were shown to amplify a 0.567-kb fragment from DNA of all the F. oxysporum f. sp. canariensis isolates tested, a rapid DNA extraction procedure was developed that led to the correct identification of 98% of the tested F. oxysporum f. sp. canariensis isolates.

© 1999 The American Phytopathological Society