Man in 't Veld
Arthur W. A. M.
Peter J. M.
First, second, third, and sixth authors: Plant Protection Service, Department of Mycology, P.O. Box 9102, NL 6700 HC Wageningen, the Netherlands; third author: Plant Protection Institute, Kostinbröd 2230, Sofia, Bulgaria; fourth author: Centraalbureau voor Schimmelcutures, Yeast Division, Julianalaan 67, NL 2628 BC Delft, the Netherlands; and fifth author: Research Institute for Plant Protection (IPO-DLO), P.O. Box 9060, 6700 GW Wageningen, the Netherlands
Go to article:
Accepted for publication 4 June 1998.
Three similar isolates of Phytophthora (Phytophthora sp-h) were obtained from diseased Spathiphyllum and Primula plants. Cultural characteristics did not fit any known description of Phytophthora species. The Phytophthora sp-h isolates are papillate, are homothallic, possess 80 to 86% amphigynous antheridia, and have a maximum temperature for growth of 36.5°C. Isozyme analysis of the Phytophthora sp-h isolates revealed a three-banded pattern with malic enzyme and a three-banded pattern with malate dehydrogenase on the second putative locus. The fastest band at both enzyme loci comigrated with the single P. nicotianae band, the slowest band comigrated with the single P. cactorum (and also P. pseudotsugae) band, and one band in between was concluded to represent the heterodimeric isozyme. The random amplified polymorphic DNA patterns of the Phytophthora sp-h isolates almost exclusively consisted of bands that were also present in either P. nicotianae or P. cactorum. Southern hybridization showed that bands specific for P. nicotianae were present as comigrating bands in the Phytophthora sp-h isolates. The same was found for species-specific bands of P. cactorum. It is concluded that the three Phytophthora sp-h isolates represent interspecific hybrids, P. nicotianae being the one parent and P. cactorum the other. Analysis of mito-chondrial DNA with restriction enzymes revealed banding patterns in all the Phytophthora sp-h isolates identical with those of P. nicotianae, confirming that indeed P. nicotianae was one of the parents.
© 1998 The American Phytopathological Society