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Diversity Within Streptomyces ipomoeae Based on Inhibitory Interactions, rep-PCR, and Plasmid Profiles

November 1998 , Volume 88 , Number  11
Pages  1,179 - 1,186

C. A. Clark , C. Chen , N. Ward-Rainey , and G. S. Pettis

First and second authors: Department of Plant Pathology and Crop Physiology, Louisiana State University Agricultural Center, Louisiana Agricultural Experiment Station, Baton Rouge 70803-1720; and third and fourth authors: Department of Biological Sciences, Louisiana State University, Baton Rouge 70803

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Accepted for publication 27 July 1998.

Streptomyces soil rot is a destructive disease of sweetpotato (Ipomoea batatas) that causes yield loss resulting from decay of the feeder root system and reduced quality due to the presence of necrotic lesions on the storage roots. It is managed by the use of resistant cultivars, but variability of the pathogen has not been previously assessed. This study compared 36 strains of the pathogen Streptomyces ipomoeae from different locations in the United States and Japan. The strains could be separated into three groups on the basis of their ability to inhibit the growth of one another in in vitro assays. Although some strains contained plasmids of approximately 18, 42, or 270 kb in size, plasmid profiles did not correspond to inhibition grouping. Fingerprinting by repetitive element-based polymerase chain reaction (rep-PCR) using outwardly facing primers for the BOX, enterobacterial repetitive intergenic consensus (ERIC), and repetitive extragenic palindromic (REP) sequences indicated relatively high genomic homogeneity within S. ipomoeae. However, cluster analysis of similarity coefficients among strains using rep-PCR data revealed clusters that correlated with the inhibition grouping. The neotype strain of S. ipomoeae had lower similarity values by rep-PCR than any of the other strains and could not be grouped by inhibitory interactions.

© 1998 The American Phytopathological Society