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Characterization of Wheatgrass-Derived Barley Yellow Dwarf Virus Resistance in a Wheat Alien Chromosome Substitution Line

August 1998 , Volume 88 , Number  8
Pages  851 - 855

Joseph M. Anderson , Dennis L. Bucholtz , Ann E. Greene , Michael G. Francki , Stewart M. Gray , Hari Sharma , Herbert W. Ohm , and Keith L. Perry

First, second, and third authors: USDA-ARS, Purdue University, 1150 Lilly Hall, West Lafayette, IN 47907-1150; fifth author: USDA-ARS, Cornell University, Ithaca, NY 14853-0331; first, fourth, sixth, and seventh authors: Agronomy Department, Purdue University, 1150 Lilly Hall, West Lafayette, IN 47907-1150; and eighth author: Botany and Plant Pathology Department, Purdue University, 1155 Lilly Hall, West Lafayette, IN 47907-1155

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Accepted for publication 27 May 1998.

Wheatgrass (Thinopyrum intermedium) possesses a high level of resistance to barley yellow dwarf virus (BYDV) subgroup I and subgroup II strains. A wheat line (P29), in which the 7D chromosome has been substituted with a group 7 chromosome from T. intermedium, was examined for the level of resistance to two subgroup I and two subgroup II BYDV strains. In P29 plants inoculated with the subgroup I PAV strains, the titer of virus in leaf and stem tissue was typically reduced 42 to 52% when compared with the BYDV-susceptible cv. Abe. P29 and ‘Abe’ had the same content of PAV in roots. These results and the absence of detectable virus in inoculated T. intermedium plants indicate that the complete resistance to subgroup I possessed by the wheatgrass has not been introgressed into P29. In contrast, P29 was completely resistant throughout the plant to the subgroup II strains, NY-RPV and NY-RMV, demonstrating that the complete resistance to subgroup II in T. intermedium was incorporated into P29. Further analysis of this resistance to NY-RPV showed that NY-RPV can replicate in mesophyll protoplasts of P29 and ‘Abe’, suggesting that this resistance is not operating at the single-cell level. Molecular marker analysis confirmed that the T. intermedium chromosome present in P29 is a different group 7 wheatgrass chromosome than that present in L1, a wheat line with BYDV resistance properties similar to those of P29.

Additional keywords: wide crosses , resistance breeding .

The American Phytopathological Society, 1998