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Serological Detection of Grapevine Virus A Using Antiserum to a Nonstructural Protein, the Putative Movement Protein

October 1997 , Volume 87 , Number  10
Pages  1,041 - 1,045

E. Rubinson , N. Galiakparov , S. Radian , I. Sela , E. Tanne , and R. Gafny

First, second, fifth, and sixth authors: Department of Virology, Agricultural Research Organization, P.O. Box 6, Bet Dagan 50250, Israel; and third and fourth authors: Virus Laboratory, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot 76100, Israel

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Accepted for publication 29 June 1997.

Grapevine virus A (GVA) is implicated in the etiology of the rugose wood disease. The coat protein (CP) and the putative movement protein (MP) genes of GVA were cloned and expressed in Escherichia coli and used to produce antisera. Both the CP and the MP were detected with their corresponding antisera in GVA-infected Nicotiana benthamiana. The MP was first detected at an early stage of the infection, 6 to 12 h after inoculation, and the CP was detected 2 to 3 days after inoculation. The CP and MP were detected by immunoblot analysis in rugose wood-affected grapevines. The MP could be detected in GVA-infected grapevines that tested negative for CP, both with CP antiserum and with a commercially available enzyme-linked immunosorbent assay kit. The study shows that detection of the nonstructural MP may be an effective means for serological detection of GVA infection in grapevines.

© 1997 The American Phytopathological Society