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Antifungal Activity of 2-Deoxy-D-Glucose on Botrytis cinerea, Penicillium expansum, and Rhizopus stolonifer: Ultrastructural and Cytochemical Aspects

July 1997 , Volume 87 , Number  7
Pages  772 - 779

Ahmed El-Ghaouth , Charles L. Wilson , and Michael Wisniewski

First author: Centre de Recherche en Horticulture, Université Laval, Quebec, G1K 7P4, Canada; and second and third authors: USDA, ARS, Appalachian Fruit Research Station, Kearneysville, WV 25530

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Accepted for publication 7 April 1997.

The effect of 2-deoxy-D-glucose on major postharvest pathogens was investigated at the ultrastructural and cytochemical level. Hyphae of Botrytis cinerea, Penicillium expansum,, and Rhizopus stolonifer grown in the absence of 2-deoxy-D-glucose were normal and showed no apparent cytological alterations. In the presence of 2-deoxy-D-glucose, however, these fungi exhibited severe cellular injuries ranging from cell wall disruption to cytoplasm disintegration. Although 2-deoxy-D-glucose caused cytoplasmic degeneration in the three fungi tested, cell wall alterations were exhibited only by B. cinerea and R. stolonifer. In the latter, the retraction of degenerated cytoplasm was often accompanied by the deposition of amorphous material in paramural spaces. Cytochemical study of fungal cell wall components showed that 2-deoxy-D-glucose caused a marked increase of chitin- and β-1,3-glucan—labeling in R. stolonifer and B. cinerea, indicating an interference of 2-deoxy-D-glucose with fungal wall biosynthesis. The observed cellular alterations indicate that 2-deoxy-D-glucose may also have affected other metabolic processes.

Additional keywords: nonmetabolizable sugars, postharvest biocontrol, sugar analogs.

The American Phytopathological Society, 1997